Blog 15: Independent Component 2 Approval
Describe in detail what you plan to do for your 30 hours.
I'm genetically engineering an enzyme. I'll be looking at the DNA of an enzyme and making a primer. I'll be site directed mutating the glgC gene. Transforming chemically competent Ecoli cells, then growing and harvesting the cells. Then I'll isolate and purify the mutated DNA. I'll cut the gene insert out of the vector which is called restriction enzyme digest. Run an agrose gel electrophoresis which separates DNA molecules based on their size. And if we see the isolated gene, we'll cut the gene out of the gel and get the DNA sequence.
Discuss how or what you will do to meet the expectation of showing 30 hours of evidence.
I'll take pictures, I'll also have pictures from doing the agrose gel electrophoresis.
And explain how what you will be doing will help you explore your topic in more depth.
This is pretty much my project, I'm genetically engineering DNA of an enzyme.
APPROVED
ReplyDelete___________
Describe in detail what you plan to do for your 30 hours.
I'm genetically engineering an enzyme. I'll be looking at the DNA of an enzyme and making a primer. I'll be site directed mutating the glgC gene. Transforming chemically competent Ecoli cells, then growing and harvesting the cells. Then I'll isolate and purify the mutated DNA. I'll cut the gene insert out of the vector which is called restriction enzyme digest. Run an agrose gel electrophoresis which separates DNA molecules based on their size. And if we see the isolated gene, we'll cut the gene out of the gel and get the DNA sequence.
Discuss how or what you will do to meet the expectation of showing 30 hours of evidence.
I'll take pictures, I'll also have pictures from doing the agrose gel electrophoresis.
And explain how what you will be doing will help you explore your topic in more depth.
This is pretty much my project, I'm genetically engineering DNA of an enzyme.