Independent Component 2
Content:
I, Christine Navarro, affirm that I completed my independent component which represents 30 hours of work.
My mentor, Mike Suseoff helped me the most during this component.
I'm genetically engineering an enzyme. I'll be looking at the DNA of an enzyme and making a primer. I'll be site directed mutating the glgC gene. Transforming chemically competent Ecoli cells, then growing and harvesting the cells. Then I'll isolate and purify the mutated DNA. I'll cut the gene insert out of the vector which is called restriction enzyme digest. Run an agrose gel electrophoresis which separates DNA molecules based on their size. And if we see the isolated gene, we'll cut the gene out of the gel and get the DNA sequence.
Site directed mutagenesis, is pretty much my Answer 1 put into place.
I, Christine Navarro, affirm that I completed my independent component which represents 30 hours of work.
My mentor, Mike Suseoff helped me the most during this component.
I'm genetically engineering an enzyme. I'll be looking at the DNA of an enzyme and making a primer. I'll be site directed mutating the glgC gene. Transforming chemically competent Ecoli cells, then growing and harvesting the cells. Then I'll isolate and purify the mutated DNA. I'll cut the gene insert out of the vector which is called restriction enzyme digest. Run an agrose gel electrophoresis which separates DNA molecules based on their size. And if we see the isolated gene, we'll cut the gene out of the gel and get the DNA sequence.
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| The smaller centrifuge |
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| Our DNA sequence for our enzyme |
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| A program we used to see if the primers I made would work or if they would be problematic. |
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| The draft of the primers I made. |
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| The machine we used to preform the transformation. |
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| The media and agar plates |
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| The unsuccessful agar plates, they grew nothing. |
Site directed mutagenesis, is pretty much my Answer 1 put into place.
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